عنوان

MRNA formation and function

0125875452

9780125875455

b553552

MRNA formation and function

[Book]

edited by Joel D. Richter.

San Diego

Academic Press

©1997.

C. Sune, P.R. Bohjanen, Y. Liu, M.A. Garcia-Blanco, and S.F. Jamison, The Tat-TAR RNP, a Master Switch That Regulates HIV-1 Gene Expression. --;C.R. Miller, S.F. Jamison, and M.A. Garcia-Blanco, HeLa Nuclear Extract: Amodified Protocol. --;J. Valcarcel, C. Martinez, and M.R. Green, Functional Analysis of Splicing Factors and Regulators. --;J.G. Patton, B.T. Dye, D.C. Barnard, and J.G. McAfee, Identification of pre-mRNA Splicing Factors and Analysis of RNA-Protein Interaction. --;G. Gilmartin, In Vitro Analysis of Mammalian Cell mRNA 3 Processing. --;J. Wilusz, Rapid Identification and Cloning of Sequence-Specific RNA Binding Proteins. --;J.S. Butler, M.W. Briggs, and A. Proweller, Analysis of Polyadenylation Phenotypes in Saccharomyces cerevisiae. --;P.D. Gershon, Poly(A) Polymerase/cap-Specific 2-O-Methyltransferase from Vaccinia Virus: Expression, Purification, Uses, and Protein-Ligand Interaction Assays. A.N. Hennigan and A. Jacobson, A Genetic Approach to Mapping Coding Region Determinants of mRNA Instability in Yeast. --;W.F. Marzluff, M.L. Whitfield, Z. Dominski, and Z.-F. Wang, Identification of the Protein Which Interacts with the 3 End of Histone mRNA. --;M. Holcik and S.A. Liebhaber, Analysis of mRNP Complexes Assembled in Vitro. --;A. Laird-Offringa, Analysis of RNA-Binding Proteins Using in Vitro Genetics. --;L.G. Andrews and J.D. Keene, Interactions of Proteins with Specific Sequences in RNA. --;C. Jain and J.G. Belasco, A Rapid Genetic Method for the Study of RNA Binding Proteins. --;P. Ansel-McKinney and L. Gehrke, Footprinting RNA-Protein Complexes with Hydroxyl Radicals. --;H. Ruan, C.Y. Brown, and D.R. Morris, Analysis of Ribosome Loading onto mRNA Species: Implications for Translational Control. --;G. Belsham, Analysis of Picornavirus Internal Ribosome Entry Site Function in Vivo. M.R. Jacobson and T. Pederson, RNA Traffic and Localization Reproted by Fluorescent Molecular Cytochemistry in Living Cells. --;A. Manoukian, Detection of mRNA in Situ: Techniques for Studying Gene Expression in Drosophila melanogaster Tissues. --;M.H. Jacob and O.C. Ikonomov, Differential Display Protocol That Preferentially Identifies mRNAs of Moderate to Low Abundance in a Microscopic System. --;Subject Index. The Tat-TAR RNP, a master switch that regulates HIV-1 gene expression --;HeLa nuclear extract: a modified protocol --;Functional analysis of splicing factors and regulators --;Identification of pre-mRNA splicing factors and analysis of RNA-protein interaction --;In Vitro analysis of mammalian cell mRNA 3' processing --;Rapid identification and cloning of sequence-specific RNA binding proteins --;Analysis of polyadenylation phenotypes in Saccharomyces cerevisiae --;Poly(A) polymerase/Cap-specific 2'-O-methyltransferase from Vaccinia virus: expression, purification, uses, and protein-ligand interaction assays --;A genetic approach to mapping coding region determinants of mRNA instability in yeast --;Identification of the protein that interacts with the 3' end of histone mRNA --;Analysis of mRNP complexes assembled in Vitro --;Analysis of RNA binding proteins using in Vitro genetics --;Interactions of proteins with specific sequences in RNA --;A rapid genetic method for the study of RNA binding proteins --;Footprinting RNA-protein complexes with hydroxyl radicals --;Analysis of ribosome loading onto mRNA species: implications for translational control --;Analysis of Picornavirus internal ribosome entry site function in Vivo --;RNA traffic and localization reported by fluorescent molecular cytochemistry in living cells. Detection of mRNA in Situ: techniques for studying gene expression in Drosophila melanogaster tissues --;Differential display protocol that preferentially identifies mRNAs of moderate to low abundance in a microscopic system.

Presents techniques geared towards the understanding of RNA metabolism. This book employs a number of different organisms and systems. It also talks about: isolation and characterization of specific RNA binding proteins; RNA metabolism and associated regulatory proteins; RNA detection and localization; and a genetic approach to RNA function.

Messenger RNA -- Research -- Methodology.

Research -- methods.

RNA, Messenger.

edited by Joel D. Richter.

[Book]

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