عنوان

Aging methods and protocols

0585344183

1280820616

1592590705

6610820619

9780585344188

9781280820618

9781592590704

9786610820610

b553206

Aging methods and protocols

[Book]

edited by Yvonne A. Barnett, Christopher R. Barnett.

Totowa, NJ

Humana Press

©2000.

(xiv, 385 pages) : illustrations

Methods in molecular medicine, 38.

1. Understanding Aging / Bernard L. Strehler --;pt. I. Study of Cell Senescence and Cell Death --;2. Use of the Fibroblast Model in the Study of Cellular Senescence / Vincent J. Cristofalo, Craig Volker and Robert G. Allen --;3. Human T-Cell Clones / Graham Pawelec --;4. Telomeres and Replicative Senescence / Hector F. Valenzuela and Rita B. Effros --;5. Detection of Molecular Events During Apoptosis by Flow Cytometry / Ruaidhri J. Carmody, Ana P. Costa-Pereira, Sharon L. McKenna, and Tom G. Cotter --;6. Raf-1 Protein Kinase Activity in T Cells from Aged Mice / Christopher J. Kirk and Richard A. Miller --;7. Identification of Differentially Expressed Genes in Young and Senescent T Cells / Andrea Engel, Mahdi Adibzadeh and Graham Pawelec --;pt. II. Age-Related Changes to Xenobiotic Metabolizing Enzyme Systems --;8. Xenobiotic-Metabolizing Enzyme Systems and Aging / Christopher R. Barnett and Costas Ioannides --;pt. III. Age-Related Changes to Defense Systems Against Biomolecule Damage --;9. Assessing Age-Related Changes in Antioxidant Status: The FRASC Assay for Total Antioxidant Power and Ascorbic Acid Concentration in Biological Fluids / Iris F.F. Benzie and John J. Strain --;10. Measurement of DNA Damage and Repair Capacity as a Function of Age Using the Comet Assay / Peter H. Clingen, Jillian E. Lowe and Michael H.L. Green --;11. Measurement of DNA Damage and Repair in Human White Blood Cells by an Immunochemical Assay / Govert P. van der Schans --;pt. IV. Age-Related Changes to Cellular Biomolecules --;12. Measurement of 8-Oxo-deoxyguanosine in Lymphocytes, Cultured Cells, and Tissue Samples by HPLC with Electrochemical Detection / Sharon G. Wood, Catherine M. Gedik, Nicholas J. Vaughan, and Andrew R. Collins --;13. Mutation and the Aging Process: Mutant Frequency at the HPRT Gene Locus as a Function of Age in Humans / Yvonne A. Barnett and Christopher R. Barnett --;14. Somatic Mutations and Aging: Methods for Molecular Analysis of HPRT Mutations / Sai-Mei Hou --;15. Assessment of Susceptibility of Low-Density Lipoprotein to Oxidation / Jane McEneny and Ian S. Young --;16. Measurement of Pentosidine in Biological Samples / Jesus R. Requena, David L. Price, Suzanne R. Thorpe, and John W. Baynes --;pt. V. Mitochondrial Morphology, Function and Mutation with Age --;17. Causes and Consequences of Damage to Mitochondria: Morphological Aspects / Jaime Miquel and Carlo Bertoni-Freddari --;18. Causes and Consequences of Damage to Mitochondria: Study of Functional Aspects by Flow Cytometry / Federico V. Pallardo, Juan Sastre, Jaime Miquel, and José Viña --;19. Analysis of Mitochondrial DNA Mutations: Deletions / Robert W. Taylor, Theresa M. Wardell, Emma L. Blakely, Gillian M. Borthwick, Elizabeth J. Brierley, and Douglass M. Turnbull --;20. Analysis of Mitochondrial DNA Mutations: Point Mutations / Robert W. Taylor, Richard M. Andrews, Patrick F. Chinnery, and Douglass M. Turnbull --;pt. VI. Immune System and Aging --;21. Assessment of T-Cell Function in the Aged: T-Cell Proliferative and T-Cell Adherence Assays / Ian Beckman --;22. Dendritic Cells in Old Age / Beatrix Grubeck-Loebenstein, Maria Saurwein-Teissl and Nikolaus Romani --;23. Age-Related Alterations to Natural Killer Cell Function / Erminia Mariani, Corona Alonso and Rafael Solana --;24. Immunogenetics and Life-Span: HLA / Derek Middleton, Martin D. Curran and Fionnuala Williams --;pt. VII. Case Studies --;25. Dietary Restriction and Life-Span Extension / Byung Pal Yu --;26. Use of Genetically Engineered Mice in Aging Research / Julie K. Andersen.

Aging is an almost universal process within biological systems, one which leads to a decline in functional capacity, disease onset, and eventually death.

Aging -- Molecular aspects -- Laboratory manuals.

Aging.

Research Design.

edited by Yvonne A. Barnett, Christopher R. Barnett.

Christopher R Barnett

Yvonne A Barnett

[Book]

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