عنوان

Cell Surface Receptors:

1461312558

9781461312550

b540789

Cell Surface Receptors:

[Book]

A Short Course on Theory and Methods

by Lee E. Limbird.

Second edition

Boston, MA

Springer US

1996

(256 pages)

1 Historical perspective --;The origin of the receptor concept --;Mass action law and occupancy theory --;The concept of efficacy --;The concept of spare receptors --;Rate theory --;Allosteric theory --;Operational models of pharmacological agonism --;Summary --;2 Methods for characterization of receptors based on receptor-mediated responses in tissue or intact cell preparations --;Characterization of receptor specificity --;Generalizations regarding the determination of equilibrium dissociation constants (KD values) for receptor-ligand interactions in intact tissue preparations --;Determination of KD values for receptor-agonist interactions, KDA --;Determination of KD values for receptor-partial agonist interactions, KDP --;Determination of KD values for receptor-antagonist interactions, KDB --;Summary --;3 Identification of receptors using direct radioligand binding techniques --;Methods --;data generation --;Choice of a radioligand --;The incubation --;Separation of bound from free radioligand --;Criteria expected for binding of D to the physiological receptor, R --;Data obtained to establish the criteria for a physiologically relevant receptor --;Summary --;4 Complex binding phenomena --;Mathematical descriptions of complex binding phenomena --;Computer-assisted analysis of complex binding phenomena --;Analysis of receptor subtypes --;Independent data consistent with the existence of receptor subtypes --;Receptor affinity states --;The ternary complex model (TCM) and expansions of the TCM --;Thermodynamic parameters of receptor-ligand interactions --;Summary --;5 The preparation and study of detergent-solubilized receptors --;General properties of biological membranes and detergent micelles --;Choice of a biological detergent --;Solubilizing receptors from biological membranes --;Methods for analysis of detergent-solubilized receptors --;Summary --;6 The topographical fate of ligand-receptor complexes as reflected by the properties of ligand binding to intact cells --;Biochemical approaches for discriminating between cell surface receptors versus intracellular receptor-ligand complexes --;Biochemical evidence consistent with recycling of cell surface receptors --;Assessment of rate constants for receptor turnover using a steady state mathematical analysis of intact cell radioligand binding data --;The heavy amino acid density-shift technique for quantitating receptor synthesis and turnover --;Summary.

Cell Surface Receptors: A Short Course on Theory and Methods, Second Edition is a primer for the study of cell surface receptors.

Medical laboratories.

Medicine.

Toxicology.

by Lee E. Limbird.

Lee E Limbird

[Book]

Y