hemopoiesis, cloning of tumors, cytogenetic and carcinogenic assays
edited by E.P. Cronkite and A.L. Carsten.
Berlin ; New York
Springer-Verlag
1980
xiii, 277 pages : illustrations ; 25 cm
I. Characterization of Progenitor Cells in Diffusion Chambers.- 1. History and Overview of the In Vivo Diffusion Chamber (DC) Culture System.- 2. Roles of CFU-S and CFU-C in Maintaining Cell Growth in Diffusion Chamber Cultures of Murine Marrow.- 3. Different Classes of Granulocyte Progenitor Cells Expressed in In Vivo Plasma Clot Diffusion Chamber Cultures.- 4. Human Granulopoietic Progenitor Cell Diversity and Regulation Studied in Diffusion Chambers.- 5. Studies on the Regulation of Diffusion Chamber Granulopoiesis.- 6. Megakaryocyte Growth in In Vitro Cultures and in Diffusion Chambers.- II. Regulatory Mechanisms in Diffusion Chamber Cell Growth.- 1. Culture of Normal Human Blood Cells in a Diffusion Chamber System II. Lymphocyte and Plasma Cell Kinetics.- 2. Marrow Culture in Diffusion Chambers in Rabbits IV. Effects of Leukocyte Products on Granulopoiesis.- 3. Host-Mediated Regulatory Activities as Measured on Bone Marrow Cells Cultured in Diffusion Chambers and In Vitro.- 4. Growth of Canine Bone Marrow and Peripheral Blood Mononuclear Cells in In Vivo Plasma Clot Diffusion Chambers in Mice: Cytopoietic Activity, GM-CFC Content, and Physical Separation.- 5. Normal and Leukemic Hematopoiesis in Diffusion Chambers: Comparison with In Vitro Culture Systems.- 6. Stimulating Factor(s) of Hematopoiesis in the Bone Marrow of Mice Administered Hydroxyurea. Effects on Diffusion Chamber Progenitor Cells (DCPC) and on Pluripotent Stem Cells (CFU-S).- III. Cell Interactions, Cytogenetics, and Methodology.- 1. Use of Diffusion Chambers for Studying Cell Interactions: Sequential Sttudies of Eosinophil Granulocytopoiesis In Vivo.- 2. An Evaluation of Host Cell Contact in the Regulation of Diffusion Chamber Hematopoiesis.- 3. Stem Cells in Human Peripheral Blood: A Method for Histologic Analysis of Colony Formation in Plasma Clot Diffusion Chambers.- 4. The Growth of Colonies of Human Bone Marrow and Tumour Cells in Soft Agar in Diffusion Chambers.- 5. Diffusion Chamber Cultures for Mutagenic and Carcinogenic Assays.- IV. Characterization of Tumour and Leukemic Cell Growth.- 1. A Colony-Forming Assay for Experimental Tumours Using the Plasma Clot Diffusion Chamber.- 2. Long-Term Culture of Monoclonal Human Tumour Cells in Diffusion Chambers.- 3. The Use of Diffusion Chambers in the Study of Tumour Immunobiology and Lymphokines.- 4. Differentiation into Granulopoiesis in Human Acute Leukemia and Blast Crisis in Chronic Myelocytic Leukemia.- 5. Isolated Leukemic Cell Influence in Normal Hemic Colony Growth.- V. Erythropoiesis in Plasma Clot Diffusion Chamber (PCDC) Culture.- VI. Summary and Conclusions.- List of Participants.