عنوان

Cell Cycle Control and Dysregulation Protocols :

0896039498

9780896039490

b538167

Cell Cycle Control and Dysregulation Protocols :

[Book]

Cyclins, Cyclin-Dependent Kinases, and Other Factors.

Antonio Giordano

Totowa

Humana Press

2004

(202 pages).

Methods in molecular biology Cell cycle control and dysregulation protocols

METHODS IN MOLECULAR BIOLOGY�"; "Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors"; "Preface"; "Contents"; "Contributors"; "PART I. ANALYSIS OF CYCLINS AND CYCLIN-DEPENDENT KINASES"; "1 The Biology of Cyclins and Cyclin-Dependent Protein Kinases: An Introduction"; "2 In Situ Immunofluorescence Analysis Immunofluorescence Microscopy"; "3 In Situ Immunofluorescence Analysis Analyzing RNA Synthesis by 5-Bromouridine-5 '-Triphosphate Labeling"; "4 Immunofluorescence Analysis Using Epitope-Tagged Proteins: In Vitro System" "5 Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteins""6 Chromatin Immunoprecipitation"; "7 Protein�Deoxyribonucleic Acid Interactions Linked to Gene Expression: Electrophoretic Mobility Shift Assay"; "8 Protein�Deoxyribonucleic Acid Interactions Linked to Gene Expression: DNase I Digestion"; "9 Protein�Deoxyribonucleic Acid Interactions Linked to Gene Expression: Ligation-Mediated Polymerase Chain Reaction"; "10 Assays for Cyclin-Dependent Kinase Inhibitors"; "11 Protein Degradation Via the Proteosome" "PART II. ANALYSIS OF THE FACTORS INVOLVED IN CELL CYCLE DEREGULATION""12 The Transformed Phenotype"; "13 A Morphologic Approach to Detect Apoptosis Based on Electron Microscopy"; "14 Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick Translation"; "PART III. CELLULAR RESPONSE TO DEOXYRIBONUCLEIC ACID DAMAGE"; "15 Induction of Deoxyribonucleic Acid Damage by Alkylating Agents"; "16 Induction of Deoxyribonucleic Acid Damage by gamma Irradiation"; "17 Ultraviolet Irradiation of Cells" "PART IV. RETROVIRIDAE-BASED VECTORS: PROTOCOLS FOR LENTIVIRAL- AND RETROVIRAL-MEDIATED GENE TRANSFER TO ENGINEER CELL CULTURE SYSTEMS""18 Transient Production of Retroviral- and Lentiviral-Based Vectors for the Transduction of Mammalian Cells"; "19 Determination of Functional Viral Titer by Drug-Resistance Colony Assay, Expression of Green Fluorescent Protein, and •-Galactoside Staining"; "20 Retroviral and Lentiviral Vector Titration by the Analysis of the Activity of Viral Reverse Transcriptase" "21 Single and Double Colloidal Gold Labeling in Postembedding Immunoelectron Microscopy""22 Multifluorescence Labeling and Colocalization Analyses"; "Index"

Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors

Cell cycle -- Laboratory manuals.

Cyclin-dependent kinases -- Laboratory manuals.

Cyclins -- Laboratory manuals.

Antonio Giordano

Antonio Giordano

Gaetano Romano

[Book]

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