عنوان

Matrix metalloproteinase protocols /

1603272984 (hbk. : alk. paper)

9781603272988 (hbk. : alk. paper)

b516652

Matrix metalloproteinase protocols /

[Book]

edited by Ian M. Clark ; consulting editors, David A. Young, Andrew D. Rowan

2nd ed

New York, N.Y. :

Humana Press,

c2010

xii, 492 p. :

ill. (some col.) ;

27 cm

Methods in molecular biology,

Springer protocols

622

1064-3745 ;

Previous ed.: 2001

Includes bibliographical references and index

Metalloproteases and the degradome / Alejandro P. Ugalde ... [et al.] -- Mouse models of MMP and TIMP function / Sean E. Gill ... [et al.] -- Expression of recombinant MMP-28 in mammalian cells / Ursula R. Rodgers and Ian M. Clark -- Expression of recombinant matrix metalloproteinases in Escherichia coli / L. Jack Windsor and Darin L. Steele -- Expression of recombinant ADAMTS in insect cells / Gavin C. Jones, Mireille N. Vankemmelbeke and David J. Buttle -- Expression and purification of membrane-type MMPs / Jing Nie and Duanqing Pei -- Refolding of TIMP-2 from Escherichia coli inclusion bodies / Richard A. Williamson -- Purification of MMPs and TIMPs / Ken-ichi Shimokawa and Hideaki Nagase -- Real-time PCR expression profiling of MMPs and TIMPs / Caroline J. Pennington and Dylan R. Edwards -- Analysis of the degradome with the CLIP-CHIP microarray / Reinhild Kappelhoff, Ulrich auf dem Keller, and Christopher M. Overall -- In situ hybridization for metalloproteinases and their inhibitors / Tiina L. Hurskainen and Suneel S. Apte -- Immunohistochemistry of MMPs and TIMPs / Yasunori Okada -- Single nucleotide polymorphism genotyping in MMP genes : the 5' nuclease assay / Ross Laxton and Shu Ye -- Methods for studying activation of matrix metalloproteinases / Vera Knäuper and Gillian Murphy -- Assay of matrix metalloproteinases against matrix substrates / Tim E. Cawston, Rachel L. Lakey, and Andrew D. Rowan -- Zymography and reverse zymography for detecting MMPs and TIMPs / Susan P. Hawkes, Hongxia Li, and Gary T. Taniguchi, with contributions from Marc A. Lafleur -- In situ zymography / Sarah J. George and Jason L. Johnson -- Near-infrared optical proteolytic beacons for in vivo imaging of matrix metalloproteinase activity / J. Oliver McIntyre, Randy L. Scherer, and Lynn M. Matrisian -- Neoepitope antibodies against MMP-cleaved and aggrecanase-cleaved aggrecan / Amanda J. Fosang ... [et al.] -- In vitro model of cartilage degradation / Wang Hui and Tim E. Cawston -- Immunoassays for collagenase-mediated cleavage of type I and II collagens / R. Clark Billinghurst, Mirela Ionescu, and A. Robin Poole -- Collagen degradation assays / Anthony P. Hollander -- Analysis of MMP-dependent cell migration and invasion / Ralf Palmisano and Yoshifumi Itoh -- Using fluorogenic peptide substrates to assay matrix metalloproteinases / Gregg B. Fields -- Kinetic analysis of the inhibition of matrix metalloproteinases : lessons from the study of tissue inhibitors of metalloproteinases / Frances Willenbrock, Daniel A. Thomas, and Augustin Amour -- Identification of cellular MMP substrates using quantitative proteomics : isotope-coded affinity tags (ICAT) and isobaric tags for relative and absolute quantification (iTRAQ) / Georgina S. Butler ... [et al.] -- Mechanism-based profiling of MMPs / Jed F. Fisher and Shahriar Mobashery

0

Biochemistry-- Technique

Metalloproteinases

Proteolytic enzymes

Extracellular Matrix Proteins

Matrix Metalloproteinases

Clark, Ian M

Rowan, Andrew D

Young, David A., (David Alan),1970-

20160427144906.0

[Book]

Y