عنوان

Guidelines for molecular analysis in archive tissues /

3642178901

9783642178900

3642178898

9783642178894

b512824

Guidelines for molecular analysis in archive tissues /

[Book]

Giorgio Stanta, editor.

New York :

Springer,

2011.

1 online resource (xvi, 323 pages) :

illustrations (some color)

Title from PDF t.p. (SpringerLink, viewed Jun 13, 2011)

Includes bibliographical references and index

Archive Tissues -- Preanalytical Time Interval (PATI) and Fixation -- Formalin-Free Fixatives -- Manual Microdissection -- Tissue Microarray (TMA) -- Laser Capture Microdissection (LCM) -- DNA Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues -- DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells) -- Fast Protocol for DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- DNA Extraction from Blood and Forensic Samples -- Restoration and Reconstruction of DNA Length -- RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- RNA Extraction from Decalcified and Non-decalcified Formalin-Fixed Paraffin-Embedded Tissues -- RNA Temperature Demodification -- MicroRNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- Quantification of Nucleic Acids -- Integrity Assessment of Nucleic Acids -- DNase Treatment of RNA -- Reverse Transcription -- General Protocol for End-Point PCR -- Quantitative End-Point PCR -- Nested-PCR -- General Protocol for Dot-Blot -- PCR-ELISA -- Quantitative Real-Time RT-PCR -- DNA Sequencing from PCR Products -- Pyrosequencing Analysis for Detection of KRAS Mutation -- Microsatellite Instability (MSI) Detection in DNA from FFPE Tissues -- General Protocol for Loss of Heterozygosity Detection -- Qualitative Methylation Status Assessment -- Quantitative Methylation Status Assessment in DNA from FFPE Tissues with Bisulfite Modification and Real-Time Quantitative MSP -- Comparative Genomic Hybridisation (CGH) -- Multiplex Ligation-dependent Probe Amplification (MLPA) -- Fluorescence In Situ Hybridization (FISH) on Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections -- Introduction on Genome-wide Expression Profiling from Formalin-Fixed Paraffin-Embedded Tissues Using Microarrays -- Introduction -- Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissues -- Rapid Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissue Samples -- Protein Extraction Protocol from Tissues Treated with Formalin-Free Fixatives -- Trichloroacetic Acid (TCA) Precipitation of Proteins -- One-Dimensional Sodium-Dodecyl-Sulfate (SDS) Polyacrylamide Gel Electrophoresis -- Visualization of Proteins -- Western Blotting -- Dot Blot -- Membrane Stripping -- Reverse-Phase Protein Microarrays -- Introduction -- Sample Preparation and In Gel Tryptic Digestion for Mass Spectrometry Experiments -- MALDI Imaging Mass Spectrometry on Formalin-Fixed Paraffin-Embedded Tissues -- Introduction -- Internal Quality Control (IQC) Organization -- Standards for Equipment and Reagents

0

Springer

978-3-642-17889-4

Guidelines for molecular analysis in archive tissues.

3642178898

SpringerLink

Pathology, Molecular.

Stanta, Giorgio.

Ohio Library and Information Network.

20120425035444.0

rda

[Book]

Y