عنوان

PCR cloning protocols /

0585403341 (electronic bk.)

0896039692 (Cloth)

0896039730 (comb. : alk. paper)

1592591779

6610821399

9780585403342 (electronic bk.)

9780896039698 (Cloth)

9780896039735 (comb. : alk. paper)

9781592591770

9786610821396

dltt

PCR cloning protocols /

[Book]

edited by Bing-Yuan Chen and Harry W. Janes.

2nd ed

2nd ed

1 online resource (xiv, 439 pages) :

illustrations.

Methods in molecular biology ;

192

Includes bibliographical references and index

Polymerase chain reaction. Basic principles and routine practice / L.A. Kolmodin and D.E. Birch -- Computer programs for PCR primer design and analysis / B.Y. Chen, H.W. Janes and S. Chen -- Single-step PCR optimization using touchdown and stepdown PCR programming / K.H. Roux -- Xl PCR amplification of long targets from genomic DNA / L.A. Kolmodin -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments / J.T. Aatsinki -- Long distance reverse-transcription PCR / V. Thiel, J. Herold and S.G. Siddell -- Increasing PCR sensitivity for amplification from paraffin-embedded tissues / A. Akalu and J.K. Reichardt -- GC-rich template amplification by inverse PCR. DNA polymerase and solvent effects / A. Moreau ... [et al.] -- PCR procedure for the isolation of trinucleotide repeats / T. Tozaki -- Methylation-specific PCR / H. Ohashi -- Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance PCR and magnetic beads / X. Huang, Z. Yuan and X. Cao -- Cloning PCR products. An overview / B. Guo and Y. Bi -- Using t4 DNA polymerase to generate clonable PCR products / K. Wang -- Enzyme-free cloning of PCR products and fusion protein expression / B.A. Neilan and D. Tillett -- Directional restriction site-free insertion of PCR products into vectors / G.J. Chen -- Autosticky PCR. Directional cloning of PCR products with performed 5' overhangs / J. Gal and M. Kalman -- A rapid and simple procedure for direct cloning of PCR products into baculoviruses / T.S. Gritsun, M.V. Mikhailov and E.A. Gould -- PCR approaches to DNA mutagenesis and recombination. An overview / B. Shen -- In-frame cloning of synthetic genes using PCR inserts / J.C. Pierce -- Megaprimer PCR / S. Barik -- PCR method for generating multiple mutations at adjacent sites / J. Adamec -- A fast polymerase chain reaction-mediated strategy for introducing repeat expansions into cag-repeat containing genes / F. Laccone -- PCR screening in signature-tagged mutagenesis of essential genes / D.E. Lehoux and R.C. Levesque -- Staggered extension process (step) in vitro recombination / A.M. Aguinaldo and F. Arnold -- Random mutagenesis by whole-plasmid PCR amplification / D. Kim and F.P. Guengerich -- PCR-based strategies to clone unknown DNA regions from known foreign integrants. An overview / E.K. Hui, P.C. Wang and S.J. Lo -- Long distance vectorette PCR (ldv PCR) / J.A. Fenton, G. Pratt and G.J. Morgan -- Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA ("cold-start method") / M. Lardelli -- Inverse PCR. cDNA cloning / S.H. Huang -- Inverse PCR. Genomic DNA cloning / A.Y. Jong ... [et al.] -- Gene cloning and expression profiling by rapid amplification of gene inserts with universal vector primers / S.H. Huang, H.Y. Wu and A.Y. Jong -- The isolation of DNA sequences flanking TN5 transposon insertions by inverse PCR / V.J. Martin and W.W. Mohn -- Rapid amplification of genomic DNA sequences tagged by insertional mutagenesis / M. Celerin and K.T. Chun -- Isolation of large-terminal sequences of bac inserts based on double-restriction-enzyme digestion followed by anchored PCR / Z.N. Yang and T.E. Mirkov -- A "step down" PCR-based technique for walking into and the subsequent direct-sequence analysis of flanking genomic DNA / Z. Zhang and S.J. Gurr -- Use of PCR in library screening. An overview / J. Zhu -- Cloning of homologous genes by gene-capture PCR / R. Mastrangeli and S. Donini -- Rapid and nonradioactive screening of recombinant libraries by PCR / M.W. King -- Rapid cDNA cloning by PCR screening (RC-PCR) / T. Takumi -- Generation and PCR screening of bacteriophage lambda sublibraries enriched for rare clones (the "sublibrary method") / M. Lardelli -- PCR-based screening for bacterial artificial chromosome libraries / Y. Yasukochi -- A 384-well microtiter-plate-based template preparation and sequencing method / L. He and K. Wang -- A microtiter-plate-based high throughput PCR product purification method / R. Smith and K. Wang

0

Annotation

Mode of access: World Wide Web

System requirements: World Wide Web browser, internet connectivity, Adobe Acrobat reader

PCR cloning protocols.

0896039692

Molecular cloning, Laboratory manuals.

Polymerase chain reaction, Laboratory manuals.

Cloning, Molecular-- methods.

Polymerase Chain Reaction-- methods.

SCI-- 029000

Chen, Bing-Yuan.

Janes, Harry W.

Ohio Library and Information Network.

20120731014536.0

rda

[Book]

Y