عنوان

Protocols for nucleic acid analysis by nonradioactive probes

089603254X

9780896032545

dltt

Protocols for nucleic acid analysis by nonradioactive probes

[Book]

edited by Peter G. Isaac.

Totowa, N.J. :

Humana Press,

c1994.

xii, 268 p. :

ill. ;

23 cm.

Methods in molecular biology ;

v. 28

Includes bibliographical references and index.

(cont) Detection of foodborne pathogens using DNA probes and a dipstick format -- Analysis of gene sequences by hybridization of PCR-amplified DNA to covalently bound oligonucleotide probes: the reverse dot blot method -- RAPD assay: a novel technique for genetic diagnostics -- Nonradioactive oligonucleotide probes for detecting products of the ligase chain reaction -- Nucleic acid sequence-based amplification (NASBA).

(cont) Preparation of chromosome spreads by root-tip meristem dissection for in situ hybridization with biotin-labeled probes -- Enzymatic treatment of plant material to spread chromosomes for in situ hybridization -- Use of biotin-labeled probes on plant chromosomes -- Direct fluorochrome-labeled DNA probes for direct fluorescent in situ hybridization to chromosomes -- Detection of dioxigenin-labeled DNA probes hybridized to plant chromosomes in situ -- Preparation of tissue sections and slides for mRNA hybridization -- Detecting mRNA in tissue sections with digoxigenin-labeled probes -- Detection of mRNA in whole mounts of mouse embryos using digoxigenin riboprobes -- PACE (Probe Assay-Chemiluminescence Enhanced): a magnetic bead assay for noncultural diagnosis of gonorrhea.

(cont) Preparation of horseradish peroxidase-labeled probes -- Random prime labeling of DNA probes with fluorescein-11-dUTP -- Labeling of oligonucleotides with fluorescein -- Hybridization of digoxigenin-labeled probes to southern blots and detection by chemiluminescence -- Southern blot hybridization of digoxigenin-labeled RNA minisatellite probes and color detection -- Hybridization and detection of digoxigenin probes on RNA blots -- Hybridization of horseradish peroxidase-labeled probes and detection by enhanced chemiluminescence -- Hybridization and detection of fluorescein-labeled DNA probes using enhanced chemiluminescence -- Hybridization of fluorescein-labeled oligonucleotide probes and enhanced chemiluminescence detection.

About nonradioactive nucleic acid detection -- Isolation of DNA from plants -- Isolation of high-molecular-weight DNA from animal cells -- Restriction enzyme digestion, gel electrophoresis and vacuum blotting of DNA to nylon membranes -- Isolation of plant RNA -- Isolation of total and polyA⁺RNA from animal cells -- Preparation of RNA gel blots -- Preparation of RNA dot blots -- Isolation of plasmids for the preparation of probes -- Production of hybridization probes by the PCR utilizing digoxigenin-modified nucleotides -- Production of DNA hybridization probes with digoxigenin-modified nucleotides by random hexanucleotide priming -- Digoxigenin labeling of RNA transcripts from multi- and single-locus DNA minisatellite probes -- Labeling of double-stranded DNA probes with biotin.

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Mode of access: World Wide Web.

Protocols for nucleic acid analysis by nonradioactive probes.

OhioLINK electronic book center

SpringerLink

Nonradioactive probes

Nucleic acid probes.

Nucleic acids-- Analysis.

Isaac, Peter G.

20081004095 002.0

[Book]

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