عنوان

Culture of animal cells :

0470528125 (cloth)

9780470528129 (cloth)

dltt

Culture of animal cells :

[Book]

a manual of basic technique and specialized applications /

R. Ian Freshney.

6th ed.

Hoboken, N.J. :

Wiley-Blackwell,

c2010.

xxxi, 732 p., [28] p. of plates :

ill. (some col.) ;

29 cm.

Includes bibliographical references and index.

1. Introduction -- 2. Biology of cultured cells -- 3. Laboratory design, layout, and equipment -- 4. Equipment and materials -- 5. Aseptic technique -- 6. Safety, bioethics, and validation -- 7. Culture vessels and substrates -- 8. Defined media and supplements -- 9. Serum-free media -- 10. Preparation and sterilization -- 11. Primary culture -- 12. Subculture and cell lines -- 13. Cloning and selection -- 14. Cell separation -- 15. Characterization -- 16. Differentiation -- 17. Transformation and immortalization -- 18. Contamination -- 19. Cryopreservation -- 20. Quantitation -- 21. Cytotoxicity -- 22. Specialized cells -- 23. Stem cells, germ cells, and amniocytes -- 24. Culture of tumor cells -- 25. Three-dimensional culture -- 26. Scale-up and automation -- 27. Specialized techniques -- 28. Training programs -- 29. Problem solving -- 30. In conclusion.

0

Cell culture, Laboratory manuals.

Tissue culture, Laboratory manuals.

Cells, Cultured.

Culture Techniques-- methods.

Freshney, R. Ian.

20110914022319.0

[Book]

Y