عنوان

Modern techniques for pathogen detection /

3527335161

3527687971

352768798X

3527687998

3527688005

9783527335169

9783527687978

9783527687985

9783527687992

9783527688005

9783527335169

dltt

Modern techniques for pathogen detection /

[Book]

edited by Jürgen Popp and Michael Bauer

1 online resource

Includes index

Includes bibliographical references at the end of each chapters and index

Unmet Medical Needs in Life-Threatening infections - Caring for the Critically Ill / Michael Bauer, Andreas Kortgen, Mervyn Singer -- Life Threatening Infections and Sepsis - Defining the Problem -- Sepsis as a "Hidden Healthcare Disaster" -- Microorganisms and Types of Infection Triggering Sepsis -- Emerging Problems Related to Resistance in Bacterial Infections -- The Role of Fungi and Viruses -- The Need for New Approaches in Diagnostics of Life-Threatening Infection and Sepsis -- Rapid and Sensitive Culture-Independent Strategies to Identify Blood Stream Infection -- Beyond Infection - Profiling the Immune Response of the Septic Host -- Host Factors Contributing to Pathogenesis of Sepsis -- References -- Identification Methods - An Overview / Oliwia Makarewicz, Claudia Stein, Wolfgang Pfister, Bettina Löffler, Mathias Pletz -- Taxonomy of Pathogenic Organisms -- Microscopic Methods -- Stains -- Autofluoresccnce -- Immunofluorescence -- In Situ Hybridization -- Dark-Field Microscopy -- Culture-Based Methods -- Blood Culture -- Automated Differentiation Systems -- API System -- Selective Agar -- Susceptibility Testing -- Other Methods -- Nucleic Acid-Based Techniques -- PCR -- Real-Time PCR -- Multiplex-PCR -- RAPD and rep-PCR -- Microaarrays -- RFLP -- Sequencing -- Ribosomal RNA Genes -- MLST -- NG Sequencing -- Serology -- Antigen-Antibody-Based Methods -- Agglutination -- Immunodiffusion -- Quantitative Immunoassays -- Automated Immunoassays -- Applications of Serological Test -- Types of Human Antibodies and What They Indicate? -- Validity of Antibodies against Opportunistic Species -- Specificity of Fungal Antigens -- Specific Urine Antigen Tests -- Biomarkers -- Conclusions and Perspectives -- References -- Nucleic Acid Amplification Techniques / Marc Lehmann, Roland P.H. Schmitz -- Introduction -- The Basic PCR Protocol -- Primer Design -- Specific versus Broad-Range Primers and the Fate of False Targeting -- Modified End-Point PCR Protocols -- Non-PCR NAT: isothermal Amplification Protocols -- Quantitative PCR -- Quantification in qPCR -- Melting Curve Analysis (MCA) -- Controls, Probing, and General Aspects of Result Interpretation -- Strategies in Amplicon Verification -- (DNA) Microarray -- Flow Cytometry -- Mass Spectrometry -- PCR Inhibitors -- Preanalyses -- Sample Volume -- Cell Lysis -- Nucleic Acid Isolation and Preparation -- Fields of PCR Application -- Sequencing -- Pyrosequencing -- Typing and Epidemiology -- Pathogen Detection in Complex Clinical Samples -- General Aspects of Result Interpretation -- Sensitivity and Specificity -- NAT versus Culture -- The Fate of Antibiotic Resistances Detection -- Microbial Trace Detection in BSI, Ascites, and Synovial Fluids -- Grown Blood Culture Media as Samples -- Upcoming Routine Solutions - Dawn of Assay Automation -- Conclusion and Perspective -- Acknowledgment -- References -- DNA Microarrays for Pathogen Detection / Holger Schulze, Maya Rubtsova, Till T. Bachmann -- Introduction -- DNA Microarrays for the Detection of Bacterial Pathogens -- Major Bacterial Diseases -- Blood Stream Infections/Sepsis -- Pneumonia -- Infectious Diarrhea -- Antibiotic Resistance Detection -- Gram-Negative Bacteria -- Gram-Positive Bacteria -- Staphylococcus aureus -- Mycobacteria -- DNA Microarrays for Virus Diagnostics -- Human Papillomaviruses -- Commercial HPV Tests -- Respiratory Viruses -- Enteric Viruses -- Hepatitis Viruses -- DNA Microarrays for Detection of Fungal Pathogens -- DNA Microarrays for Parasite Diagnostics -- Malaria -- Waterborne Parasites -- Other Parasites -- Conclusion an Outlook -- References -- MALDI-ToF / Stefan Zimmermann -- Introduction -- MALDI-ToF Technology -- Bacterial Identification Using Mass Spectrometry -- Culture-Independent Rapid Identification of Clinical Pathogens -- Antibiotic Susceptibility Testing Using Mass Spectrometry -- References -- IR and Raman Spectroscopy for Pathogen Detection / Ute Münchberg, Sandra Kloß, Dragana Kusic, Susann Meisel, Ralf Heinke, Stephan Stöckel, Petra Rösen, Jürgen Popp -- Introduction -- Bulk Analysis -- IR and NIR Absorption Spectroscopy -- Raman Spectroscopy with Excitation in the NIR Region -- Resonance Raman Spectroscopy -- Excitation with Visible Wavelengths -- UV-Resonance Raman Spectroscopy -- Surface-Enhanced Raman Spectroscopy -- The SERS Effect -- Analysis of Bulk Bacteria Samples with SERS -- Single-Cell Analyses -- Micro-Raman Spectroscopy with Excitation in the Visible or NIR Region -- Surface-Enhanced Raman Spectroscopy on Single Cells -- Conclusion and Outlook -- References -- FISH / Graeme N. Forrest, Jwan Mohammadi, Shahrzad Mohammadi -- Introduction -- FISH Techniques -- PNA FISH -- Original PNA FISH Platform -- PNA FISH Flow Cytometry -- Beacon-Based FISH (bbFISH) -- Clinical Implications of PNA FISH -- Gram-Positive Organisms -- Staphylococci -- Enterococci -- Gram-Negative Probes -- Candida -- Beta Hemolytic Streptococci -- Mycobacteria -- Helicobacter pylori -- Other Bacteria -- Parasites -- Summary and Outlook -- References -- Conclusions / Sibyll Pollok, Karina Weber, Michael Bauer, Jürgen Popp

0

9783527687978

Pathogenic microorganisms-- Detection.

TEC-- 010000

Bauer, Michael

Popp, Jürgen

Ohio Library and Information Network.

20160603081227.7

pn

[Book]

Y