عنوان

PCR technology :

143984805X (hardcover : alk. paper)

9781439848050 (hardcover : alk. paper)

dltt

PCR technology :

[Book]

current innovations /

edited by Tania Nolan, Stephen A. Bustin

3rd ed. /

editors, Tania Nolan, Stephen A. Bustin

Boca Raton :

CRC Press, Taylor & Francis,

2013

xxi, 435 p., [12] p. of plates :

ill. (some col.) ;

27 cm

Includes bibliographical references and index

PCR's simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that provides a critical and timely reference point for anyone wishing to use this technology. Topics in this forward-thinking volume include: The purification and handling of PCR templates, The effect of the manufacture and purification of the oligonucleotide on PCR behavior, Optimum buffer composition, Probe options, The design and optimization of qPCR assays, Issues surrounding the development and refinement of instrumentation, Effective controls to protect against uncertainties due to reaction variability, Covering all aspects of PCR and real-time PCR, the book contains detailed protocols that make it suitable as both a reference and an instruction manual. Each chapter presents detailed guidelines as well as helpful hints and tips supplied by authors who are recognized experts in their fields. In addition to descriptions of current technology and best practices, the book also provides information about new developments in the PCR arena. Book jacket

Polymerase chain reaction-- Methodology

Nucleic Acids-- analysis

Polymerase Chain Reaction-- methods

Bustin, Stephen A

Bustin, Stephen A.,1954-

Nolan, Tania

20140317083019.0

[Book]

Y