عنوان

Calorimetry /

0128029064

9780128029060

dltt

Calorimetry /

[Book]

Edited by Andrew L. Feig

xix, 493 pages, 16 unnumbered pages of plates :

illustrations (some color) ;

24 cm

Methods in enzymology,

volume 567

0076-6879 ;

Includes bibliographical references and indexes

Isothermal titration calorimetry measurements of metal ions binding to proteins -- Assess coupled protein folding and binding through temperature-dependent isothermal titration calorimetry -- Fragment-based screening for enzyme inhibitors using calorimetry -- Measuring multivalent binding interactions by isothermal titration calorimetry -- Calorimetric and spectroscopic analysis of the thermal stability of short duplex DNA-containing sugar and base-modified nucleotides -- Calorimetric quantification of cyclodextrin-mediated detergent extraction for membrane-protein reconstitution -- Extending ITC to kinetics with KinlTC -- Measuring the kinetics of molecular association by isothermal titration calorimetry -- Isothermal titration calorimetry to characterize enzymatic reactions -- Avoiding buffer interference in ITC experiments: a case study from the analysis of entropy-driven reactions of glucose-6-phosphate dehydrogenase -- ITC methods for assessing buffer/protein interactions from the perturbation of steady-state kinetics: a reactivity study of homoprotocatechuate 2,3-dioxygenase -- Modern analysis of protein folding by differential scanning calorimetry -- A guide to differential scanning calorimetry of membrane and soluble proteins in detergents -- Orthogonal methods for characterizing the unfolding of therapeutic monoclonal antibodies: differential scanning calorimetry, isothermal chemical denaturation, and intrinsic fluorescence with concomitant static light scattering -- IATC, DSC, and PPC analysis of reversible and multistate structural transition of cytochrome c -- The complementairy of the loop to the stem in DNA pseudoknots gives rise to local TAT base-triplets -- A high-throughput biological calorimetry core: steps to startup, run, and maintain a multiuser facility

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Calorimetry

Feig, Andrew L

20160325015305.0

rda

[Book]

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