Electron paramagnetic resonance investigations of biological systems by using spin labels, spin probes, and intrinsic metal ions,
[Book]
edited by Peter Z. Qin, Kurt Warncke
Part A /
First edition
xx, 684 pages, 16 unnumbered pages of plates :
illustrations (some color) ;
24 cm
Methods in enzymology,
volume 563
0076-6879 ;
Includes bibliographical references and indexes
Development and application of spin traps, spin probes, and spin labels -- Site-directed spin labeling of RNA by postsynthetic modification of 2'-amino groups -- Gd³⁺ spin labeling for measuring distances in biomacromolecules : why and how? -- Cu²⁺ as an ESR probe of protein structure and function -- Genetically encoded spin labels for in vitro and in-cell EPR studies of native proteins -- Genetic incorporation of the unnatural amino acid p-acetyl phenylalanine into proteins for site-directed spin labeling -- A straightfoward approach to the analysis of double electron-electron reosnance data -- Computer modeling of spin labels -- NASNOX, PRONOX, and ALLNOX -- mtsslSuite : probing biomolecular conformation by spin-labeling studies -- Full atom simulations of spin label conformations
Rapid-scan EPR of nitroxide spin labels and semiquinones -- Fourier deconvolution methods for resolution enhancement in continuous-wave EPR spectroscopy -- Multifrequency pulsed EPR and the characterization of molecular dynamics -- Resolution and characterization of chemical steps in enzyme catalytic sequences by using low-temperature and time-resolved, full spectrum EPR spectroscopy in fluid cryosolvent and frozen solution systems -- 230/115 GHz electron paramagnetic resonance/double electron-electron resonance spectroscopy -- CW-EPR spectral simulations : solid state -- CW-EPR spectral simulations : slow-motion regime -- Quantitative interpretation of multifrequency multimode EPR spectra of metal containing proteins, enzymes, and biomimetic complexes -- Pulse double-resonance EPR techniques for the study of metallobiomolecules -- Mapping the structure of metalloproteins with RIDME -- Structural characterization of the catalytic sites of mononuclear nonheme fe hydroxylases using ²H-ESEEM -- Pulsed EPR in the study of drug binding in cytochrome P450 and NOS -- EPR methods for biological Cu(II) : L-band CW and NARS