edited by Rajyalakshmi Luthra, Rajesh R. Singh, and Keyur P. Patel, Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Third edition.
New York :
Humana Press,
[2016]
Methods in molecular biology,
Springer protocols
1392
1064-3745 ;
Preceded by: Clinical applications of PCR / edited by Y.M. Dennis Lo, Rossa W.K. Chiu, K.C. Allen Chan. c2006.
Includes bibliographical references and index.
This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routineclinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successfulMethods in Molecular Biologyseries, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Clinical Applications of PCR, Third Editionserves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.