edited by Juan Carlos Lacal, Rosario Perona, James Feramisco
248 pages :
illustrations ;
25 cm
Methods and tools in biosciences and medicine
Includes bibliographical references and index
1. Needle Microinjection: A Brief History / J. Feramisco, R. Perona and J. C. Lacal -- 2. Microinjection of Macromolecules into Mammalian Cells in Culture / R. Perona, F. Dolfi and J. Feramisco / [et al.] -- 3. The Use of Plasmid Microinjection to Study Specific Cell Cycle Phase Transitions / E. S. Knudsen -- 4. Combining Microinjection with Microanalytical Methods to Investigate Regulation of Metabolic Pathways / J. Manchester and J. C. Lawrence, Jr. -- 5. Import of Stably Folded Proteins into Peroxisomes / P. A. Walton -- 6. Study of the Function of Microtubule Proteins / R. Armas-Portela and J. Avila -- 7. Cytoskeleton Regulation by Rho Proteins / R. Kozma, S. Ahmed and L. Lim -- 8. Cytoskeletal Regulation by Rho-p21 Associated Kinases: Analysis of ROK and PAK Function by Plasmid Microinjection / T. Leung, E. Manser and L. Lim -- 9. Use of Microinjection to Study Apoptosis and its Prevention / F. Kohlhuber, H. Hermeking and D. Eick -- 10. Role of the InsPase and Casuperscript 2+] Entry / R. S. Mathias and H. E. Ives -- 11. Microinjection of Xenopus laevis Oocytes: A Model System / J. C. Lacal -- 12. Xenopus laevis Oocytes as a Model for Studying the Activation of Intracellular Kinases / A. Carnero and J. C. Lacal -- 13. Signal Transduction in Xenopus laevis Oocytes by Ras Oncoproteins and Lipid Metabolites / A. Carnero and J. C. Lacal -- 14. The Multiple Roles of Mos During Meiosis / M. S. Murakami and G. F. Vande Woude -- 15. Use of the Xenopus Oocyte System to Study RNA Transport / L. A. Allison -- 16. Functional Expression of G Protein-Coupled Receptors in Xenopus laevis Oocytes / P. de la Pena and F. Barros -- 17. Expression of Glucose Transporters in Xenopus laevis Oocytes: Applications for the Study of Membrane Transporter Function and Regulation / M. J. Seatter and G. W. Gould -- 18. DNA Injection into Xenopus laevis Embryos as a Tool to Study Spatial Gene Activity / M. Kuhl, M. Walter and J. Clement / [et al.]
19. Transformation of Nematodes by Microinjection / S. Hashmi, G. Hashmi and R. Gaugler -- Guide to Solutions -- Guide to Protocols -- Troubleshooting Guide