عنوان

JAK-STAT signalling :

162703241X

9781627032414

b405618

JAK-STAT signalling :

[Book]

methods and protocols /

edited by Sandra E. Nicholson and Nicos A. Nicola.

New York :

Humana Press,

c2013.

xi, 320 p. :

ill. ;

26 cm.

Methods in molecular biology,

Springer protocols

967

1064-3745 ;

Includes bibliographical references and index.

Analysis of Janus tyrosine kinase phosphorylation and activation / Jeremy A. Ross, Georgialina Rodriguez, and Robert A. Kirken -- Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins / Claude Haan and Serge Haan -- In vitro JAK kinase activity and inhibition assays / Jeffrey J. Babon and James M. Murphy -- Quantitative analysis of JAK binding using isothermal titration calorimetry and surface plasmon resonance / Jeffrey J. Babon -- Determination of protein turnover rates in the JAK/STAT pathway using a radioactive pulse-chase approach / Anna Dittrich, Elmar Siewert, and Fred Schaper -- Designing RNAi screens to identify JAK/STAT pathway components / Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler -- The use of JAK-specific inhibitors as chemical biology tools / Christopher J. Burns, David Segal, and Andrew F. Wilks -- Methods for detecting mutations in the human JAK2 gene / Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green -- Detection of activated STAT proteins / Jean-Patrick Parisien and Curt M. Horvath -- Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents / Serge Haan and Claude Haan -- Flow cytometric analysis of STAT phosphorylation / Jane Murphy and Gabrielle L. Goldberg -- Acetylation of endogenous STAT proteins / Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer -- Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining / Reza Khorooshi and Trevor Owens -- Nuclear trafficking of STAT proteins visualized by live cell imaging / Velasco Cimica and Nancy C. Reich -- Characterization of STAT self-association by analytical ultracentrifugation / Nikola Wenta and Uwe Vinkemeier -- Constitutively active STAT5 constructs / Lynn M. Heltemes-Harris and Michael A. Farrar -- Analysis of suppressor of cytokine signalling (SOCS) gene expression by real-time quantitative PCR / Tatiana B. Kolesnik and Sandra E. Nicholson -- Detection of endogenous SOCS1 and SOCS3 proteins by immunoprecipitation and western blot analysis / Jian-Guo Zhang and Sandra E. Nicholson -- In vitro ubiquitination and cytokine signaling components / Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw -- Production and crystallization of recombinant JAK proteins / Isabelle S. Lucet and Rebecca Bamert -- Bacterial expression, purification, and crystallization of tyrosine phosphorylated STAT proteins / Florence Baudin and Christoph W. Müller.

0

Cellular signal transduction, Laboratory manuals.

Cytokines, Laboratory manuals.

Protein kinases, Laboratory manuals.

Janus Kinases-- metabolism, Laboratory Manuals.

Signal Transduction, Laboratory Manuals.

STAT Transcription Factors-- metabolism, Laboratory Manuals.

Nicholson, Sandra E.

Nicola, Nicos,1950-

20130211091617.0

[Book]

Y