Roles and functions of plant mineral nutrients -- Plant growth and cultivation -- Protocols for growing plant symbioses: mycorrhiza -- Protocols for growing plant symbioses: rhizobia -- Plant cell suspension cultures -- Soil analysis using visible and near infrared spectroscopy -- Mineral composition analysis: measuring anion uptake and anion concentrations in plant tissues -- Multielement plant tissue analysis using ICP spectrometry -- Mapping element distributions in plant tissues using synchrotron X-ray fluorescence techniques -- Flux measurements of cations using radioactive tracers -- Ion flux measurements using the MIFE techique -- Sampling and analysis of phloem sap -- Methods for xylem sap collection -- Plant single cell sampling -- Measurements of cytosolic ion concentrations in live cells -- Real time measurement of cytoplasmic ions -- Large-scale plant ionomics -- Applications of high-throughput plant phenotyping to study nutrient use efficiency.