Digital images are data : and should be treated as such / Douglas W. Cromey -- Epi-fluorescence microscopy / Donna J. Webb and Claire M. Brown -- Live-cell migration and adhesion turnover assays / J. Lacoste, K. Young, and Claire M. Brown -- Multifluorescence confocal microscopy : application for a quantitative analysis of hemostatic proteins in human venous valves / Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill -- Colocalization analysis in fluorescence microscopy / Jeremy Adler and Ingela Parmryd -- A time-lapse imaging assay to study nuclear envelope breakdown / Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman -- Light sheet microsocopy in cell biology / Raju Tomer, Khaled Khairy, and Philipp J. Keller -- Image-based high-throughput screening for inhibitors of angiogenesis / Lasse Evensen, Wolfgang Link, and James B. Lorens -- Intravital microscopy to image membrane trafficking in live rats / Andrius Masedunskas ... [et al.] -- Imaging non-fluorescent nanoparticles in living cells with wavelength-dependent differential interference contrast microscopy and planar illumination microscopy / Wei Sun, Lehui Xiao, and Ning Fang -- Laser scanning cytometry : principles and applications-an update / Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz -- Laser capture microdissection for protein and NanoString RNA analysis / Yelena Golubeva ... [et al.] -- Viewing dynamic interactons of proteins and a model lipid membrane with atomic force microscopy / Anthony S. Quinn ... [et al.] -- Mica functionaliztion for imaging of DNA and protein-DNA complexes with atomic force microscopy / Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri Lyubchenko -- Measuring the elastic properties of living cells with atomic force microscopy indentation / Joanna L. MacKay and Sanjay Kumar -- Atomic force microscopy functional imaging on vascular endothelial cells / Lilia A. Chtcheglova and Peter Hinterdorfer -- Porosome : the secretory nanomachine in cells / Bhanu P. Jena -- Stereology and morphometry of lung tissue / Christian Mühlfeld, Lars Knudsen, and Matthias Ochs -- A novel combined imaging/morphometrical method for the analysis of human sural nerve biopsies for clinical diagnosis / Michele A. vonTurkovich ... [et al.] -- Correlative light-electron microscopy as a tool to study in vivo dynamics and ultrastructure of intracellular structures / Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini -- Photooxidation technology for correlative light and electron microscopy / Claudia Meisslitzer-Ruppitsch ... [et al.] -- Electron microscopy of endocytic pathways / Carmen Ranftler ... [et al.] -- Morphological analysis of autophagy / Keisuke Tabata ... [et al.] -- Cytochemical detection of peroxisomes and mitochondria / Nina A. Bonekamp ... [et al.] -- Histochemical detection of lipid droplets in cultured cells / Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto -- Environmental scanning electron microscopy in cell biology / J.E. McGregor ... [et al.] -- Environmental scanning electron microscopy gold immunolabeling in cell biology / Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi -- High-pressure freezing for scanning transmission electron tomography analysis of cellular organelles / Paul Walther, Eberhard Schmid, and Katharina Höhn -- MALDI imaging mass spectrometry for direct tissue analysis / Stephan Meding and Axel Walch.