High-Performance Liquid Chromatography of Peptides and Proteins :
[Book]
Separation, Analysis, and Conformation
editors, Hodges, Robert S.
First edition
Boca Raton, FL
CRC Press,
2017.
text file, PDF
Cover Title Page Copyright Page Preface Table of Contents SECTION I: PRACTICAL ASPECTS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY Properties of Peptides/Proteins and Practical Implications Standard Chromatographic Conditions for Size-Exclusion, Ion-Exchange, Reversed-Phase and Hydrophobic Interaction Chromatography Preventive Maintenance and Troubleshooting LC Instrumentation Tools and Techniques to Extend LC Column Lifetimes Mobile Phase Preparation and Column Maintenance HPLC Guard Columns On-Line Derivatization of Silica Supports for Regeneration of Reversed-Phase Columns. SECTION II: THEORETICAL ASPECTS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYHPLC Terminology: Practical and Theoretical High-Performance Liquid Chromatography of Peptides and Proteins: General Principles and Basic Theory High-Performance Liquid Chromatography of Peptides and Proteins: Quantitative Relationships for Isocratic and Gradient Elution of Peptides and Proteins SECTION III: SIZE-EXCLUSION CHROMATOGRAPHY Requirement for Peptide Standards to Monitor Ideal and Non-Ideal Behavior in Size- Exclusion Chromatography High-Perfonnance Size-Exclusion Chromatography of Proteins. Size-Exclusion Chromatography of ProteinsSize-Exclusion High-Performance Liquid Chromatography of Integral Membrane Proteins and the Effect of Detergents on Immunological Activity A Reversed-Phase Trace Enrichment Technique for the Loading of Size-Exclusion HPLC Columns SECTION IV: ION-EXCHANGE CHROMATOGRAPHY The Use of Peptide Standards for Monitoring Ideal and Non-Ideal Behavior in Cation-Exchange Chromatography Ion-Exchange High-Performance Liquid Chromatography of Peptides Use of Ion-Exchange Cartridges for Batch Extraction in the Purification of Pituitary Peptides. High-Performance Ion-Exchange Chromatography of ProteinsHigh-Performance Ion-Exchange Chromatography of Proteins: Application to the Purification of Enzymes Anion Exchange High-Performance Liquid Chromatography of Viral Proteins Sample Preparation and Application of Different HPLC Methods for the Separation of Hydrophobic Membrane Proteins Cation-Exchange High-Performance Liquid Chromatography: Group Separation of Acidic and Basic Proteins Using Volatile Solvents Separation of Protein Isoforms: An Ion-Exchange HPLC Separation of Bovine and Cardiac Troponin Complexes. Assessment of Steroid Receptor Polymorphism by High-Performance Ion-Exchange ChromatographySECTION V: REVERSED-PHASE CHROMATOGRAPHY Reversed-Phase Packings for the Separation of Peptides and Proteins by Means of Gradient Elution High-Performance Liquid Chromatography Commercially Available Columns and Packings for Reversed-Phase HPLC of Peptides and Proteins Requirement for Peptide Standards to Monitor Colunm Performance and the Effect of Column Dimensions, Organic Modifiers, and Temperature in Reversed-Phase Chromatography.