عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
387P
انگلیسی
Isolation, culture and diffrentiation of bone marrow-derived stromal cells into osteoblasts and isolation and generation of osteoblasts of rat calvariae for optimization and their effectiveness in cell therapy of legg calve perthes disease
مقایسه نتایج استخراج، کشت و تمایز سلول های بنیادی مغز استخوان موش صحرایی(wistar rat) به سلول استئوبلاست و خالص سازی سلول های استئوبلاست استخوان کالواریا جهت بهینه سازی و ارزیابی کارایی آنها در سلول درمانی بیماری پرتس
[Dissertation]
Nesa Milan
نسا میلان
Tehran University of Medical Sciences, Faculty of Medicine
2022
62p
Doctor of medicine (MD)
2022/07/30
Introduction: Legg-Calvé-Perthes disease is an idiopathic hip disorder characterized by ischemic necrosis of the growing femoral head due to the temporary interruption in blood supply to the femoral head.The existing treatments cannot reliably prevent femoral head abnormalities. Regenerative medicine is a new type of treatment that uses stem cells to replace lost cells. The ability of stem cells to differentiate and renew themselves makes them unique. Using bone marrow, which contains osteogenic cells, may help treat Perthes disease. Currently, mesenchymal stem cells (MSCs) are the first source of cells for regenerative medicine research and therapeutic applications. Osteoblasts are bone-forming cells derived from mesenchymal stem cells. Mesenchymal cells can be converted into osteoblast cells responsible for bone repair and regeneration, thus resulting in effective treatments. In this study, we will compare the results of utilizing bone marrow stem cells and osteoblast cells and determine which cells are suitable for cell therapy. Materials and methods: Our experimental study involves two types of cell lines:1) Extraction, purification, and differentiation of bone marrow mesenchymal stem cells into osteoblasts.2) extraction and purification of osteoblast cells. 3- Flow cytometry, alkaline phosphatase activity, and alizarin red staining were performed as evaluation for confirmation. -Flow cytometry evaluation: In order to assess whether the extracted cells are MSCs, flow cytometry evaluation will be performed in passage 4, with positive markers CD90, CD44, CD105 and negative markers CD29, CD45. -Alkaline phosphatase activity:After culture, alkaline phosphatase is highly expressed during the early stages of bone differentiation. As a result, it is evaluated as a key indicator for early osteogenesis on days 0, 7, 14, and 21. -Alizarin red staining: It is performed on days 7, 14 and 21 following culture to detect the mineralized nodules. Results:Differentiation media were used to differentiate BMMSCs to osteoblasts in passage 4. In the flow cytometry analysis, CD90 and CD44 were positive, while CD29 and CD45 were negative, which confirms the presence of BMMSCs. Alizarin red staining demonstrated increasing mineralization extent up to the 21st day. Between differentiated BMMSCs, alkaline phosphatase activity was at the highest level on the 21st day. However, osteoblasts significantly exhibited the highest level of Alkaline phosphatase activity among all cell lines. conclusion: The highest osteogenic capacity was found in purified osteoblasts. Although differentiation of mesenchymal stem cells into osteoblast-like cells showed efficient results, using osteoblasts has demonstrated more osteogenic potential and can be a promising cell type for the treatment of Perthes.
legg calve
bone
cell therapy
سلول های بنیادی مغز استخوان
, Author
, Author
Milan. Nesa
میلان، نسا
, Thesis advisor
, Thesis advisor
, Thesis advisor
, Consulting advisor
, Consulting advisor
Nabian, Mohammad hossein
نبیان، محمدحسین
Hasan Nezhad, Zahra
حسن نژاد، زهرا
oriyadi zanjani, Leila
اوریادی زنجانی، لیلا
Tehran University of Medical Sciences, Faculty of Medicine
ایران
20230425
387 pardis
مطالعه متن کتاب p
TL
276903
a
Y
