عنوان

Protein chromatography

9781493964123)electronic bk.(

Protein chromatography

edited by Dermot Walls and Sin?ش‌ad T. Loughran

2nd ed.

New York, N.Y.

Humana Press ;Springer,

c2017

1 online resource )x, 426 p.( , ill. )some col.(.

Springer protocols.

Methods in molecular biology,1940-6029 ;v. 1485.

Series: Springer Protocols )Series(

Series: Springer protocols )Series(

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1485.

Synopsis of proteins and their purification /Dermot Walls, Gary Cooney, and Sin?ad T. Loughran --Gel-filtration chromatography /Ciaran ??'F??g??in, Philip M. Cummins, and Brendan F. O'Connor --Immunoaffinity chromatography : concepts and applications /Jenny Fitzgerald ... ]et al.[ --Avoiding poteolysis during protein purification /Barry J. Ryan and Gary T. Henehan --Scale-up of protein purification : downstream processing issues /John Joseph Milne --Phage display : a powerful technology for the generation of high-specificity affinity reagents from alternative immune sources /William J. J. Finlay ... ]et al.[ --Protein stability : enhancement and measurement /Ciar??n ??'F??g??in --Tagging recombinant proteins to enhance solubility and aid purification /Sin?ش‌ad T. Loughran and Dermot Walls --Storage and lyophilization of pure proteins /Ciar??n ??'F??g??in and Keith Colliton --Differential precipitation and solubilization of proteins /Barry J. Ryan and Gemma K. Kinsella --Ion-exchange chromatography : basic principles and application /Philip M. Cummins, Keith D. Rochfort, and Brendan F. O'Connor --Protein quantitation and analysis of purity /Eva M. Campion, Sin?ش‌ad T. Loughran, and Dermot Walls --Purification of proteins fused to maltose-binding protein /Mario Lebendiker and Tsafi Danieli --Purification of plyhistidine-tagged proteins /Sin?ش‌ad T. Loughran, Ronan T. Bree, and Dermot Walls --Purification of antibodies using affinity chromatography /Elaine Darcy ... ]et al.[ --Optimized generation of high-affinity, high-specificity single-chain Fv Antibodies from multi-antigen immunized chickens /William J.J. Finlay ... ]et al.[ --Measuring protein-protein interactions using biacore /Paul Leonard ... ]et al.[ --Hydrophobic interaction chromatography /Brendan F. O'Connor and Philip M. Cummins --Fast protein liquid chromatography /Ashkan Madadlou, Siobhan O'Sullivan, and David Sheehan --Clinical proteomics : liquid chromatography-mass spectrometry )LC-MS( purification systems /Michael Henry and Paula Meleady --Strategies for the purification of membrane proteins /Sin?ش‌ad M. Smith --Antimicrobial peptide production and purification /Srinivas Suda, Des Field, and Niall Barron --Lectin affinity chromatography )LAC( /Brendan F. O'Connor, Donal Monaghan, and Jonathan Cawley. This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.

Includes bibliographical references and index.

methods and protocols

Proteomics

Proteins, Analysis

Chromatographic analysis

Proteins, analysis

Chromatography, methods

Walls, Dermot.

Loughran, Sin?ش‌ad T.

English Book