عنوان

Plant epigenetics

1489977066

Plant epigenetics

edited by Igor Kovalchuk

human press

2016

Methods in molecular biology,1064-3745 ;v. 1456

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;

Series: Springer protocols

Chromatin immunoprecipitation protocol for histone modifications and protein-DNA binding analyses in Arabidopsis /Wanhui You, Stiٶephane Pien, and Ueli Grossniklaus --Chromatin conformation capture-based analysis of nuclear architecture /Stefan Grob and Ueli Grossniklaus --Meta-analysis of genome-wide chromatin data /Julia Engelhorn and Franziska Turck --Localization of miRNAs by in situ hybridization in plants using conventional oligonucleotide probes /Sara Herniٶandez-Castellano, Geovanny I. Nic-Can, and Clelia De-la-Pe?na --Combined bisulfite restriction analysis )COBRA( assay for the analysis of locus-specific changes in methylation patterns /Andriy Bilichak and Igor Kovalchuk --Analysis of global genome methylation using the cytosine-extension assay /Andriy Bilichak and Igor Kovalchuk --In situ analysis of DNA methylation in plants /Palak Kathiria and Igor Kovalchuk --Analysis of DNA hydroxymethylation using colorimetric assay /Andrey Golubov and Igor Kovalchuk --Analysis of DNA cytosine methylation patterns using methylation-sensitive amplification polymorphism )MSAP( /Mariٶia iٶAngeles Guevara ]and others[ --Differentially methylated region-representational difference analysis )DMR-RDA( : a powerful method to identify DMRs in uncharacterized genomes /Pavlina Sasheva and Ueli Grossniklaus --Analysis of small RNA populations using hybridization to DNA tiling arrays /Martine Boccara ]and others[ --Northern blotting techniques for small RNAs /Todd Blevins --Stem-loop qRT-PCR for the detection of plant microRNAs /Erika Varkonyi-Gasic --Profiling new small RNA sequences /Masayuki Tsuzuki and Yuichiro Watanabe --Small RNA library preparation and illumina sequencing in plants /Andriy Bilichak, Andrey Golubov, and Igor Kovalchuk --Bioinformatics analysis of small RNA transcriptomes : the detailed workflow /Slava Ilnytskyy and Andriy Bilichak --Increasing a stable transformation efficiency of Arabidopsis by manipulating the endogenous gene expression using virus-induced gene silencing /Andriy Bilichak and Igor Kovalchuk --Random oligonucleotide-primed synthesis assay for the quantification of DNA strand breaks /Andriy Bilichak and Igor Kovalchuk --Profiling transposable elements and their epigenetic effects in non-model species /Christian This volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different degrees of complexity, and describe bioinformatics approaches for data processing and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vita

Includes bibliographical references and index.

methods and protocols

Plant genetic reg

Epi

Epigenesis., fast, )OCoLC(fst

Plant genetic regulation., fast, )OCoLC(fst

Kovalchuk, Igor

English Book