عنوان

Plant-pathogen interactions

9781627039857 )alk. paper(

Plant-pathogen interactions

edited by Paul Birch, Dundee Effector Consortium, Cell and Molecular Sciences, The James Hutton Institute, Dundee, UK, Division of Plant Sciences, College of Life Sciences, University of Dundee, Dundee )at JHI(, UK, John T. Jones, Cell and Molecular Sciences, The James Hutton Institute, Dundee, UK, Jorunn I.B. Bos, Cell and Molecular Sciences, The James Hutton Institute, Dundee, UK, Division of Plant Sciences, College of Life Sciences, University of Dundee, Dundee, UK

Second edition.

New York

Springer

2014

xiii, 306 pages : illustrations )some college(

Methods in molecular biology,1064-3745 ;1127

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1127.

Galaxy as a Platform for Identifying Candidate Pathogen Effectors --Bioinformatic analysis of expression data to identify effector candidates --Two-dimensional data binning for the analysis of genome architecture in filamentous plant pathogens and other eukaryotes --On The Statistics Of Identifying Candidate Pathogen Effectors --High-throughput imaging of plant immune responses --In vivo protein-protein interaction studies with BiFC: conditions, cautions and caveats --Particle bombardment-mediated transient expression to identify localization signals in plant disease resistance proteins and target sites for the proteolytic activity of pathogen effectors --Purification of fungal haustoria from infected plant tissue by flow cytometry --Functional Characterisation of Nematode Effectors in Plants --Silencing of aphid genes by feeding on stable transgenic Arabidopsis thaliana --Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids --A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana --Simple quantification of in planta fungal biomass --Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum --DIGE-ABPP by click chemistry: Pairwise comparison of serine hydrolase activities from the apoplast of infected plants --A Simple and Fast Protocol for the Protein Complex Immunoprecipitation )Co-IP( of Effector Host Protein Complexes --An Arabidopsis and Tomato Mesophyll Protoplast System for fast Identification of early MAMP-triggered Immunity-Suppressing Effectors --Production of RXLR effector proteins for structural analysis by X-ray crystallography --The Dos and Donts of effectoromics --Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice --A Bacterial Type III Secretion Based Delivery System for Functional Assays of Fungal Effectors in Cereals --Capture arrays to annotate resistance genes in plant genomes and to accelerate plant resistance gene discovery.

Includes bibliographical references and index.

methods and protocols

Plant-pathogen relationships^aMolecular aspects--Research--Methodology

Plant diseases^aMolecular aspects--Research--Methodology

Birch, Pau

Jones, John T.

Bos, Jorunn I. B.

English Book