عنوان

Biotechnology of plant secondary metabolism

9781493933914 )alk. paper(

Biotechnology of plant secondary metabolism

edited by Arthur Germano Fett-Neto, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

Springer

2016

xi, 180 pages : illustrations )some color(

Methods in molecular biology,1064-3745 ;1405

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1405.1064-3745

Series: Springer protocols )Series(1949-2448

This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry.--Source other than Library of Congress.

Elicitation approaches for withanolide production in hairy root culture of Withania somnifera )L.( Dunal /Ganeshan Sivanandhan ]and others[ --Stimulant paste preparation and bark streak tapping technique for pine oleoresin extraction /Thanise Nogueira Feuller ]and others[ --Modified protocol for high-quality RNA extraction from oleoresin-producing adult pines /Jaulio Caesar de Lima ]and others[ --Collection of apoplastic fluids from Arabidopsis thaliana leaves /Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier --From plant extract to a cDNA encoding a glucosyltransferase candidate : proteomics and transcriptomics as tools to help elucidate saponin biosynthesis in Centella asiatica /Fernanda de Costa ]and others[ --Production of recombinant caffeine synthase from guarana )Paullinia cupana var. sorbilis( in Escherichia coli /Flaavia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera --Heterologous expression and characterization of mimosinase from Leucaena leucocephala /Vishal Singh Negi and Dulal Borthakur --Production of aromatic plant terpenoids in recombinant baker's yeast /Anita Emmerstorfer-Augustin and Harald Pichler --Purification of a recombinant polyhistidine-tagged glucosyltransferase using immobilized metal-affinity chromatography )IMAC( /Fernanda de Costa ]and others[ --Western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes /Morten Egevang J▓rgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier --Laser capture microdissection : avoiding bias in analysis by selecting just what matters /Maarcia R. de Almeida and Martina V. Streomvik --Analytical and fluorimetric methods for the characterization of the transmembrane transport of specialized metabolites in plants /Inaes Carqueijeiro ]and others[ --Protoplast transformation as a plant-transferable transient expression system /Patraicia Duarte ]and others[ --Design, construction, and validation of artificial MicroRNA vectors using agrobacterium-mediated transient expression system /Basdeo Bhagwat ]and others[ --Knockdown of polyphenol oxidase gene expression in potato )Solanum tuberosum L.( with artificial MicroRNAs /Ming Chi ]and others[.

Includes bibliographical references and index.

methods and protocols

Plant cells and tissues^aLaboratory manuals

Plants^ametabolism

Plants^agenetics

Secondary Metabolism

Biotechnology

Laboratory Manuals

Plant cells and tissues.^afast--)OCoLC(fst01065318

Laboratory manuals.^afast--)OCoLC(fst01920776

Fett-Neto, Arthur Germano

1967

English Book