عنوان

RNA scaffolds

9781493927296 )alk. paper(

RNA scaffolds

edited by Luc Ponchon, University of Paris Descartes, Paris, France

New York

Springer Science]Business Media

c2015

xiii, 231 pages : illustrations )some color(

Methods in Molecular Biology,1064-3745 ;1316

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;1316.

Series: Springer protocols )Series(1949-2448

Method to predict the 3D structure of an RNA scaffold /Xiaojun Xu and Shi-Jie Chen --Post-crystallization improvement of RNA crystal diffraction quality /Jinwei Zhang and Adrian R. Ferrae-D'Amarae --Expression and purification of RNA-protein complexes in Escherichia coli /Margueritte El Khouri and others --Production of homogeneous recombinant RNA using a tRNA scaffold and hammerhead ribozymes /Frank H.T. Nelissen, Hans A. Heus, and Sybren S. Wijmenga --In vivo production of small recombinant RNAs embedded in a 5S rRNA-derived protective scaffold /Victor G. Stepanov and George E. Fox --Detection of RNA-protein interactions using tethered RNA affinity capture /Hidekazu Iioka and Ian G. Macara --Universal method for labeling native RNA in live bacterial cells /Irina Smolina and Natalia Broude --Live cell imaging using riboswitch-spinach tRNA fusions as metabolite-sensing fluorescent biosensors /Colleen A. Kellenberger, Zachary F. Hallberg, and Ming C. Hammond --RNA scaffold : designed to co-localize enzymes /ZJU China Team )iGEM 2012( and Ming Chen --Artificial ligase ribozymes isolated by a "Design and Selection" strategy /Shigeyoshi Matsumura and Yoshiya Ikawa --Engineering aptazyme switches for conditional gene expression in mammalian cells utilizing an in vivo screening approach /Charlotte Rehm, Benedikt Klauser, and Jeorg S. Hartig --Aptazyme-based riboswitches and logic gates in mammalian cells /Yoko Nomura and Yohei Yokobayashi --Design and characterization of topological small RNAs /Jack Hassall and others --Folding RNA-protein complex into designed nanostructures /Tomonori Shibata and others --Simple method for constructing RNA triangle, square, pentagon by tuning interior RNA 3WJ angle from 60└ to 90└ or 108└ /Emil F. Khisamutdinov and others --RNA-mediated CdS-based nanostructures /Vinit Kumar and Anil Kumar --Effective method for specific gene silencing in Escherichia coli using artificial small RNA /Geunu Bak and others.

"This volume provides techniques on recent developments that use RNA scaffolds as molecular tools. RNA Scaffolds: Methods and Protocols guides readers through methods on various domains as molecular biology, nanotechnologies, and structural biology. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Scaffolds: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field."--Back cover.

Includes bibliographical references and index.

methods and protocols

RNA^aLaboratory manuals

Synthetic biology^aLaboratory manuals

RNA^aLaboratory Manuals

Cell Nucleus Structures^aLaboratory Manuals

Laboratory Manuals

RNA.^afast--)OCoLC(fst01086244

Synthetic biology.^afast--)OCoLC(fst01762461

Laboratory manuals.^afast--)OCoLC(fst01920776

Ponchon, Luc

1974

English Book