عنوان

Plant cell expansion

9781493919017)alk. paper(

Plant cell expansion

edited by Josae M. Estevez

springer

c2015

xi, 231 pages : illustrations

Methods in molecular biology,1064-3745 ;1242

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;1242.1604-3745

Series: Springer protocols )Series(1949-2448

Penium margaritaceum as a model organism for cell wall analysis of expanding plant cells /Maja G. Rydahl ]and others[ --Using chemical genomics to study cell wall formation and cell growth in Arabidopsis thaliana and Penium margaritaceum /N. Worden ]and others[ --Optimized method for growing in vitro Arabidopsis thaliana pollen tubes /Cecilia Borassi ]and others[ --Imaging of calcium dynamics in pollen tube cytoplasm /Maraia Laura Barberini and Jorge Muschietti --Live imaging of root hairs /Silvia M. Velasquez, Jose R. Dinneny, and Josae M. Estevez --Improved ROS measurement in root hair cells /Paola Silvina Denita Juaarez, Silvina Mangano, and Josae M. Estevez --Root hair assay to expedite cell death research /Joanna Kacprzyk and Paul F. McCabe --Vacuolar staining methods in plant cells /David Scheuring ]and others[ --Live cell imaging of FM4-64, a tool for tracing the endocytic pathways in Arabidopsis root cells /Adeline Rigal, Siamsa M. Doyle, and Staephanie Robert --Salt-stress regulation of root system growth and architecture in Arabidopsis seedlings /Lina Duan, Jose Sebastian, and Jose R. Dinneny --Quantification of fluorescent reporters in plant cells /Michael Pound, Andrew P. French, and Darren M. Wells --Live cell imaging of the cytoskeleton and cell wall enzymes in plant cells /Arun Sampathkumar and Raymond Wightman --Using the split-ubiquitin yeast two-hybrid system to test protein-protein interactions of transmembrane proteins /Logan Bashline and Ying Gu --Activation tag screening for cell expansion genes in Arabidopsis thaliana /Chaowen Xiao and Charles T. Anderson --BiFC for protein-protein interactions and protein topology : discussing an integrative approach for an old technique /Giovanni Stefano, Luciana Renna, and Federica Brandizzi --N-Glycosylation and plant cell growth /Christiane Veit, Ulrike Vavra, and Richard Strasser --Peptide separation methodologies for in-depth proteomics /Sajad Majeed Zargar ]and others[ --Structural and mechanical characterization of growing Arabidopsis plant cell walls /Friederike Saxe, Ingo Burgert, and Michaela Eder.

This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists.--Source other than the Library of Congress.

Includes bibliographical references and index.

methods and protocols

Plant cell walls^aLaboratory manuals

Plants^aLaboratory manuals

Plant Cells

Cell Enlargement

Cell Size

Plant Physiological Phenomena

Laboratory Manuals

Plant cell walls.^afast--)OCoLC(fst01065315

Plants.^afast--)OCoLC(fst01065823

Laboratory manuals.^afast--)OCoLC(fst01920776

Estevez, Josae M.

English Book