عنوان

siRNA design

9781627031189 )alk. paper(

siRNA design

edited by Debra J. Taxman

New York

Humana Press,

c2013

xiii, 392 p. : ill. )some col.(.

Methods in molecular biology,1064-3745 ;942

Springer protocols,1949-2448

Series: Methods in molecular biology )Clifton, N.J.( ;v. 942.1064-3745

Series: Springer protocols.1949-2448

What parameters to consider and which software tools to use for target selection and molecular design of small interfering RNAs /Olga Matveeva --Methods for selecting effective siRNA target sequences using a variety of statistical and analytical techniques /Shigeru Takasaki --Designing functional siRNA with reduced off-target effects /Yuki Naito and Kumiko Ui-Tei --Design and screening of siRNAs against highly structured RNA targets /Neda Nasheri, John Paul Pezacki, and Selena M. Sagan --Engineering small interfering RNAs by strategic chemical modification /Jesper B. Bramsen and J▓rgen Kjems --Design, selection, and evaluation of highly specific and functional siRNA incorporating unlocked nucleobase analogs /Narendra Vaish and Pinky Agarwal --Design, preparation and evaluation of asymmetric small interfering RNA for specific gene silencing in mammalian cells /Chanil Chang ... ]et al.[ --Design of nuclease-resistant fork-like small interfering RNA )fsiRNA( /Elena L. Chernolovskaya and Marina A. Zenkova --Designing dual-targeting siRNA duplexes having two active strands that combine siRNA and MicroRNA-like targeting /Pal S╡trom --Strategies for designing and Validating immunostimulatory siRNAs /Michael P. Gantier --Designing efficient and specific endoribonuclease-prepared siRNAs /Vineeth Surendranath ... ]et al.[ --Short hairpin RNA-mediated gene silencing /Luke S. Lambeth and Craig A. Smith --Design of lentivirally expressed siRNAs /Ying Poi Liu and Ben Berkhout --Bifunctional short hairpin RNA )bi-shRNA( : design and pathway to clinical application /Donald D. Rao ... ]et al.[ --Design and chemical modification of synthetic short shRNAs as potent RNAi triggers /Anne Dallas and Brian H. Johnston --Production and application of long dsRNA in mammalian cells /Katerina Chalupnikova, Jana Nejepinska, and Petr Svoboda --Design of RNAi reagents for invertebrate model organisms and human disease vectors /Thomas Horn and Michael Boutros --Construction of shRNA expression plasmids for silkworm cell lines using single-stranded DNA and Bst DNA polymerase /Hiromitsu Tanaka --Designing effective amiRNA and multimeric amiRNA against plant viruses /Muhammad Fahim and Philip J. Larkin --Downregulation of plant genes with miRNA-induced gene silencing /Felipe Fenselau de Felippes.

Includes bibliographical references and index.

methods and protocols

Small interfering RNA

RNA, Small Interfering^agenetics--Laboratory Manuals

Gene Silencing^aLaboratory Manuals

Gene Targeting^amethods--Laboratory Manuals

Laboratory Manuals

Taxman, Debra J

English Book