عنوان

Fluorescent protein-based biosensors

1627036210 )alk. paper(

Fluorescent protein-based biosensors

edited by Jin Zhang and Qiang Ni, , Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA, Robert H. Newman, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA, Department of Biology, North Carolina A&T State University, Greensboro, NC, USA

xi, 251 pages : illustrations )some colored(

Methods in molecular biology,1064-3745 ;1071

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1071.1064-3745

Series: Springer protocols )Series(1949-2448

Design and application of genetically encodable biosensors based on fluorescent proteins /Robert H. Newman and Jin Zhang --Introduction to fluorescence imaging techniques geared towards biosensor applications /J. Goedhart, Mark A. Hink, and Kees Jalink --Quantitative measurement of Cap2]s and Znp2]s in mammalian cells using genetically encoded fluorescent biosensors /J. Genevieve Park and Amy E. Palmer --Detecting cAMP with an epac-based FRET sensor in single living cells /J. Klarenbeek and Kees Jalink --Analysis of compartmentalized cAMP : a method to compare signals from differently targeted FRET reporters /Alessandra Stangherlin ... ]et al.[ --Genetically encoded fluorescent biosensors for live cell imaging of lipid dynamics /Moritoshi Sato --Live-cell imaging of cytosolic NADH-NADp]s redox state using a genetically encoded fluorescent biosensor /Yin Pun Hung and Gary Yellen --Measuring membrane voltage with microbial rhodopsins /Adam E. Cohen and Daniel R. Hochbaum --Imaging the activity of Ras superfamily GTPase proteins in small subcellular compartments in neurons /Ana F. Oliveira and Ryohei Yasuda --Imaging kinase activity at protein scaffolds /Maya T. Kunkel and Alexandra C. Newton --Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells /Sohum Mehta and Jin Zhang --Genetically encoded FRET indicators for live-cell imaging of histone acetylation /Kazuki Sasaki and Minoru Yoshida --Genetically encoded fluorescent biosensors for live-cell imaging of MT1-MMP protease activity /Mingxing Ouyang, Shaoying Lu, and Yingxiao Wang --Biosensor imaging in brain slice preparations /Marina Polito, Pierre Vincent, and Elvire Guiot --Optical calcium imaging using DNA-encoded fluorescence sensors in transgenic fruit flies, Drosophila melanogaster /Shubham Dipt, Thomas Riemensperger, and Andrae Fiala --Multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase a dynamics in parallel /Nwe-Nwe Aye-Han and Jin Zhang --FRET and BRET-based biosensors in live cell compound screens /Katie Herbst Robinson, Jessica R. Yang, and Jin Zhang --Integrating fluorescent biosensor data using computational models /Eric C. Greenwald, Renata K. Polanowska-Grabowska, and Jeffrey J. Saucerman.

Includes bibliographical references and index.

methods and protocols

Biosensors^aLaboratory manuals

Fluorescent probes^aLaboratory manuals

Biosensing Techniques^amethods--Laboratory Manuals

Green Fluorescent Proteins^agenetics--Laboratory Manuals

Signal Transduction^aLaboratory Manuals

Laboratory Manuals.^amesh

Zhang, Ji

Ni, Qiang

Newman, Robert H.

English Book