عنوان

Recombinant protein production in yeast

9781617797699 )alk. paper(

Recombinant protein production in yeast

edited by Roslyn M. Bill

New York

Humana Press,

c2012

xii, 248 p. : ill. )some col.(

Methods in molecular biology,1940-6029 ;866

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;866.

Series: Springer protocols.

Optimising yeast as a host for recombinant protein production )review( / Nicklas Bonander and Roslyn M. Bill -- Which yeast species shall I choose? : Saccharomyces cerevisiae versus Pichia pastoris )review( / Richard A.J. Darby ... ]et al.[ -- Preparation of Pichia pastoris expression plasmids / Christel Logez ... ]et al.[ -- Preparation of Saccharomyces cerevisiae expression plasmids / David Drew and Hyun Kim -- Codon optimisation for heterologous gene expression in yeast / Kristina Hedfalk -- Yeast transformation to generate high-yielding clones / Mohammed Jamshad and Richard A.J. Darby -- Screening for high-yielding Pichia pastoris clones : the production of G protein-coupled receptors as a case study / Shweta Singh ... ]et al.[ -- Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins / David Drew and Hyun Kim -- The effect of antifoam addition on protein production yields / Sarah J. Routledge and Roslyn M. Bill -- Setting up a bioreactor for recombinant protein production in yeast / Sarah J. Routlledge and Michelle Clare -- The implementation of a design of experiments strategy to increase recombinant protein yields in yeast )review( / Nagamani Bora ... ]et al.[ -- Online analysis and process control in recombinant protein production )review( / Shane M. Palmer and Edmund R.S. Kunji -- Monitoring the biomass accumulation of recombinant yeast cultures : offline estimations of dry cell mass and cell counts / Shane M. Palmer and Edmund R.S. Kunji -- Online monitoring of biomass accumulation in recombinant yeast cultures / Shane M. Palmer and Edmund R.S. Kunji -- Optimising Pichia pastoris induction / Zharain Bawa and Richard A.J. Darby -- Optimizing Saccharomyces cerevisiae induction regimes / David Drew and Hyun Kim -- Large-scale production of membrane proteins in Pichia pastoris : the production of G protein-coupled receptors as a case study / Shweta Singh ... ]et al.[ -- Large-scale production of secreted proteins in Pichia pastoris / Nagamani Bora -- Disruption of yeast cells to isolate recombinant proteins / Mohammed Jamshad and Richard A.J. Darby.

Includes bibliographical references and index.

methods and protocols

Recombinant proteins^aLaboratory manuals

Yeast fungi^aBiotechnology--Laboratory manuals

Recombinant Proteins^abiosynthesis--Laboratory Manuals

Yeasts^aLaboratory Manuals

Pichia^aLaboratory Manuals

Saccharomyces cerevisiae^aLaboratory Manuals

Bill, Roslyn M.

1967

English Book