عنوان

Phospho-proteomics

9781603278331 )hbk. : alk. paper(

Phospho-proteomics

edited by Marjo de Graauw

New York, NY

Humana Press,

c2009

xi, 328 p. : ill.

Methods in molecular biology,1064-3745 ;527

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;v. 527.

Series: Springer protocols.

A high-resolution two dimensional gel- and pro-Q DPS-based proteomics workflow for phosphoprotein identification and quantitative profiling / Ganesh K. Agrawal and Jay J. Thelen -- Differential phosphoprotein labelling )DIPPL( using p32sP and p33sP / Aviva M. Tolkovsky and Andreas Wyttenbach -- Identification of oxidative stress-induced tyrosine phosphorylated proteins by immunoprecipitation and mass spectrometry / Ma Carmen Duran, Hong-Lin Chan, and John F. Timms -- Enrichment and characterization of phosphopeptides by immobilized metal affinity chromatography )IMAC( and mass spectrometry / Tine E. Thingholm and Ole N. Jensen -- The use of titanium dioxide micro-columns to selectively isolate phosphopeptides from proteolytic digests / Tine E. Thingholm and Martin R. Larsen -- Enrichment and separation of mono- and multiply phosphorylated peptides using sequential elution from IMAC prior to mass spectrometric analysis / Tine E. Thingholm , Ole N. Jensen, and Martin R. Larsen -- Use of stable isotope labeling by amino acids in cell culture )SILAC( for phophotyrosine protein identification and quantitation / Guoan Zhang and Thomas A. Neubert -- Hydrophilic interaction chromatography for fractionation and enrichment of the phosphoproteome / Dean E. McNulty and Roland S. Annan -- SILAC for global phosphoproteomic analysis / Genaro Pimienta, Raghothama Chaerkady, and Akhilesh Pandey -- Quantitative phospho-proteomics based on soluble nanopolymers / Anton Iliuk and W. Andy Tao -- Profiling the tyrosine phosphorylation state using SH2 domains / Kevin Dierck ... ]et al.[ -- An overview of the qualitative analysis of phosphoproteins by mass spectrometry / Philip R. Gafken -- The analysis of phosphoproteomes by selective labelling and advanced mass spectrometric techniques / Angela Amoresano ... ]et al.[ -- On-line liquid chromatography electron capture dissociation for the characterization of phosphorylation sites in proteins / Steve M.M. Sweet and Helen J. Cooper -- Quantification of protein phosphorylation by ]mu[LC-ICP-MS / Ralf Kreuger, Nico Zinn, and Wolf D. Lehmann -- Reverse-phase diagonal chromatography for phosphoproteome research / Kris Gevaert and Joeel Vandekerckhove -- Chemical tagging strategies for mass spectrometry-based phospho-proteomics / Alexander Leitner and Wolfgang Lindner -- Antibody array platform to monitor protein tyrosine phosphorylation in mammalian cells / Alicia S. Chung and Y. Eugene Chin -- Protein tyrosine kinase characterization based on fully automated synthesis of )phospho(peptide arrays in microplates / W. Carl Saxinger -- Kinome profiling using peptide arrays in eukaryotic cells / Kaushal Parikh, Maikel P. Peppelenbosch, and Tita Ritsema -- ProMoST : a tool for calculating the pI and molecular mass of phosphorylated and modified proteins on two-dimensional gels / Brian D. Halligan -- Kinase-specific preiction of protein phosphorylation sites / Martin L. Miller and Nikolaj Blom -- Reconstructing regulatory kinase pathways from phosphopeptide data : a bioinformatics approach / Lawrence G. Puente, Robin E.C. Lee, and Lynn A. Megeney.

Includes bibliographical references and index.

methods and protocols

Phosphoproteins

Phosphoproteins

Phosphorylation

Proteomics^amethods

Protaeomique.^afmesh

Phosphorylation.^afmesh

Phosphore^aMaetabolisme.--ram

Graauw, Marjo de

1978

English Book