عنوان

Enzyme engineering

9781627032926 )alk. paper(

Enzyme engineering

edited by James C. Samuelson

New York

Humana Press ;Springer,

c2013

xi, 252 p. : ill. )some col.(

Methods in molecular biology,1064-3745 ;978

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;v. 978.

Series: Springer protocols )Series(

Tripartite fusion system for the selection of protein variants with increased stability in vivo /Linda Foit and James C.A. Bardwell --Determining enzyme kinetics via isothermal titration calorimetry /Neil A. Demarse ... ]et al.[ --GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria /Olga Paley ... ]et al.[ --Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties /Sarah K. Baxter ... ]et al.[ --TAL effector nuclease )TALEN( engineering /Ting Li and Bing Yang --In vitro evolution of enzymes /Misha V. Golynskiy ... ]et al.[ --Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo /Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington --Reconstructing evolutionary adaptive paths for protein engineering /Megan F. Cole ... ]et al.[ --Oligonucleotide recombination enabled site-specific mutagenesis in bacteria /Bryan M. Swingle --FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants /Eric R. Geertsma --Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes /Hidehiko Hirakawa and Teruyuki Nagamune --Gene synthesis by assembly of deoxyuridine-containing oligonucleotides /Romualdas Vaisvila and Jurate Bitinaite --Protein engineering : single or multiple site-directed mutagenesis /Pei-Chung Hsieh and Romualdas Vaisvila --Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination /Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish --Promiscuity-based enzyme selection for rational directed evolution experiments /Sandeep Chakraborty ... ]et al.[ --Rational protein sequence diversification by multi-codon scanning mutagenesis /Jia Liu and T. Ashton Cropp --Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter /Jian-Wei Liu and David L. Ollis --In vitro directed evolution of enzymes expressed by E. coli in microtiter plates /Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis.

Includes bibliographical references and index.

methods and protocols

Enzymes^aBiotechnology

Enzymes^aIndustrial applications

Protein engineering

Enzymes^aLaboratory Manuals

Protein Engineering^amethods--Laboratory Manuals

Biomedical Engineering^amethods--Laboratory Manuals

Samuelson, James C.

1968

English Book