edited by Erik Dassi, Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Mattarella, Trento, Italy
Second edition.
New York
Humana press
2016
xiii, 353 pages : illustrations )some color( ; 27 cm
Methods in molecular biology,1064-3745 ;v. 1358
Springer protocols
Series: Methods in molecular biology )Clifton, N.J.( ;v. 1358,1064-3745
Series: Springer protocols )Series(,1949-2448
Regulation of gene expression /Alessandro Quattrone and Erik Dassi --Computational approach for the discovery of protein-RNA networks /Domenica Marchese, Carmen Maria Livi, and Gian Gaetano Tartaglia --Transcriptional regulation with CRISPR/Cas9 effectors in mammalian cells /Hannah Pham, Nicola A. Kearns, and Renae Maehr --Studying the translatome with polysome profiling /Paola Zuccotti and Angelika Modelska --Exploring ribosome positioning on translating transcripts with ribosome profiling /Pieter Spealman ]and others[ --Studying isoform-specific mRNA recruitment to polyribosomes with Frac-seq /Rocio T. Martinez-Nunez and Jeremy R. Sanford --Use of the pBUTR reporter system for scalable analysis of 3' UTR-mediated gene regulation /Arindam Chaudhury and Joel R. Neilson --Comprehensive identification of RNA-binding proteins by RNA interactome capture /Alfredo Castello ]and others[ --Identifying RBP targets with RIP-seq /Hans-Herman Wessels ]and others[ --PAR-CLIP : a method for transcriptome-wide identification of RNA binding protein interaction sites /Charles Danan, Sudhir Manickavel, and Markus Hafner --Profiling the binding sites of RNA-binding proteins with nucleotide resolution using iCLIP /FX Reymond Sutandy, Andrea Hildebrandt, and Julian Keonig --Pipeline for PAR-CLIP data analysis /Marvin Jens --Capture and identification of miRNA targets by biotin pulldown and RNA-seq /Shen Mynn Tan and Judy Lieberman --Identification of miRNA-target RNA interactions using CLASH /Aleksandra Helwak and David Tollervey --Genome-wide analysis of A-to-I RNA editing /Yiannis A. Savva, Georges St. Laurent, and Robert A. Reenan --Nucleotide-level profiling of m5C RNA methylation /Tennille Sibbritt ]and others[ --Probing N 6-methyladenosine )m6A( RNA modification in total RNA with SCARLET /Nian Liu and Tao Pan --Genome-wide identification of alternative polyadenylation events using 3'T-fill /Stefan Wilkening, Vicent Pelechano, and Lars M. Steinmetz --Genome-wide profiling of alternative translation initiation sites /Xiangwei Gao, Ji Wan, and Shu-Bing Qian --Genome-wide study of mRNA isoform half-lives /Joseph V. Geisberg and Zarmik Moqtaderi --Visualizing mRNA dynamics in live neurons and brain tissues /Hye Yoon Park and Minho Song --Single-molecule live-cell visualization of pre-mRNA splicing /Robert M. Martin ]and others[.
Includes bibliographical references and index.
Genetic regulation^aLaboratory manuals
Messenger RNA^aLaboratory manuals
Cellular control mechanisms^aLaboratory manuals
Bioinformatics
Gene Expression Regulation
RNA Processing, Post-Transcriptional
Computational Biology
Laboratory Manuals
Bioinformatics.^afast--)OCoLC(fst00832181
Cellular control mechanisms.^afast--)OCoLC(fst00850278