عنوان

High throughput protein expression and purificationmethods and protocols. ]CD[,High throughput protein expression and purification )Online : Serials Solutions(. ]CD[

1066

High throughput protein expression and purificationmethods and protocols. ]CD[

Totowa, N.J. : Springer, distributor[

Humana Press ; ]London :

c2009

xii, 322 p. : ill. )some col.( ; 27 cm.

Methods in molecular biology ;894.

Includes bibliographical references and index

High-throughput protein production )HTTP( : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt -- Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley -- Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath -- Flexi vector cloning / Paul G. Blommel ... ]et al.[ -- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens -- The polymerase incomplete primer extension )PIPE( method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley -- A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... ]et al.[ -- "System 84" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart -- Automated 69-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni2+-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery -- E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... ]et al.[High-throughput protein production )HTTP( : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt -- Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley -- Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath -- Flexi vector cloning / Paul G. Blommel ... ]et al.[ -- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens -- The polymerase incomplete primer extension )PIPE( method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley -- A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... ]et al.[ -- "System 84" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart -- Automated 69-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni2+-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery -- E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... ]et al.[

High throughput protein expression and purification )Online : Serials Solutions(. ]CD[

Separation Methodology ، Proteins

genetics ، Proteins

isolation & purification ، Proteins

، Gene Expression

methods ، Proteomics

، Electronic books

TI

edited by Sharon A. Doyle

AU (nnA norahS).A norahS ,elyoD

CO Springer Protocols

SE

SE Methods in molecular biology )Clifton, N.J.( ;

ش دیداری شنیداری‌بخ